Application of DNA markers for tomato breeding for slow ripening and yellow leaf curl virus resistance
Updated on 09:26, Monday, 12/05/2014 (GMT+7)
On 8th May 2014, a seminar under the framework of the project "Application of DNA markers for tomato breeding for slow ripening and yellow leaf curl virus resistance (YLCV)” was held at Hanoi University of Agriculture (HUA). The Project has been conducted by the Department of Molecular Biology and Applied Biotechnology, the Faculty of Biotechnology, HUA, since 2011.
\r\n
Tested hybrid tomato
\r\n
Since the project inception, three hundred and nineteen tomato accessions have been collected and evaluated for agronomic traits such as growth pattern, yield, quality, and YLCV resistance. The genes related to slow ripening (rin) and TYLC resistance (Ty1, Ty2, Ty3) have been investigated by using DNA markers. Breeding for new tomato varieties has been carried out by means of crossing and Marker-Assisted Selection. Results showed that of 319studied tomato accessions 7 were found carrying gene related to slow ripening genes (rin), 14 carryingTy1, 12 carrying Ty3,and 10 carrying both Ty1 and Ty3 . Three hybrid lines, viz. CC1, CC2 and CC3,showed high heterosis, slow ripening and resistance against YLCV. The hybrid lines have been tested in several provinces. It was revealed that the average yield was about 60-65 tons/ha and their fruits were slow ripening with a long shelf life of at least 2 months after harvesting under normal preservation. These results may contribute to the breeding of tomatoes with high yields and potential for long term preservation. It would bring many social and economic benefits for farmers and consumers.
\r\n
\r\n
Department of Molecular Biology and Applied Biotechnology, Faculty of Biology